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As per available reports about 10 relevant journals, 15 Conferences, 30 workshops are presently dedicated exclusively to immunoprecipitation and about 2,070 articles are being published Immunoprecipitation.
Immunoprecipitation is defined as the technique of precipitating an antigenic protein from the solution by using an antibody that specifically binds to that particular protein. Antibodies used should be attached to a solid substrate so that the coloured reaction product obtained can be easily identified. The basic principal of this technique is to isolate and concentrate a particular protein from a sample containing many different proteins. Immunoprecipitation is a keystone technique that is used by molecular biologists to analyze protein–protein interactions, DNA-protein interactions. There are various types of immunoprecipitation i.e. Individual protein immunoprecipitation, Chromatin immunoprecipitation, RNA immunoprecipitation, Protein complex immunoprecipitation.
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Scope and Importance
Immunoprecipitation (IP) is the small-scale affinity purification of antigens using a specific antibody and is one of the most widely used methods for antigen purification and detection. This approach enables researchers to:identify the activation status of proteins, determine post-translational protein modifications, measure the molecular weight of a given protein, capture protein-binding molecules in the study of protein-protein and protein-nucleic acid interactions
Individual protein immunoprecipitation used for biochemical characterization of proteins involves the use of antibody that is particular for a known protein so as to isolate that particular protein out of solution. Chromatin immunoprecipitation is used to determine the location of DNA binding sites on the genome for a particular protein of interest. The advantage of chromatin immunoprecipitation is that protein–DNA interactions inside the nucleus of living cells or tissues can be identified. Protein complex immunoprecipitation also called as co-immunoprecipitation is based on the selection of the antibody that targets a known protein only so that by targeting this known protein with an antibody it may become possible to pull the entire protein complex out of solution and thereby unknown proteins in the complex can be identified.
This concise analysis discusses the diagnostic technologies market by types which include DNA methylation and ChIP (Chromatin Immunoprecipitation) technology. Market size estimates and forecasts for the period 2010 to 2018 has been given for each segment and sub-segment, in terms of USD million, using 2010 as the base year.
The global epigenetics drugs and diagnostic technologies market has also been segmented geographically into four regions namely North America, Europe, Asia-Pacific, and Rest of the World (RoW). Market size and forecast has been provided for each of these regions. A detailed qualitative analysis of the factors responsible for driving and restraining the growth of the epigenetics market and future opportunities are provided in the report.
2.Johnson & Johnson
5.Merck & Co.
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This page was last updated on 12th Sep, 2015
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