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Trypsinization is defined as the process of dissociating a cell using trypsin. Trypsin is a proteolytic enzyme which breaks down proteins and digests adherent cells in the vessel in which they are cultured. Upon addition to animal cell culture, it breaks down the proteins and thus enabling the cells to get adheres to the vessel. However cell surface proteins are cleaved sometimes due to the proteolytic activity of trypsin which leads to dysregulation of the cell functions. To overcome that it is necessary to add trypsin inhibitors like serum and divalent cations like calcium and magnesium can be added. Serum is usually added to the container once cells have detached and cell detachment can be confirmed by observing under a microscope.
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Scope and Importance:
Trypsinization is often done to permit passage of the cells to a new container, observation for experimentation, or reduction of the degree of confluence in the flask by removal of a percentage of the cells. This process of cell culture or tissue culture requires a method to dissociate the cells from the container and each other. Trypsin, an enzyme commonly found in the digestive tract, can be used to "digest" the proteins that facilitate adhesion to the container and between cells. For experimental purposes, cells are often cultivated in containers that take the form of plastic flasks or plates. In such flasks, cells are provided with growth medium comprising the essential nutrients required for proliferation, and the cells adhere to the container and each other as they grow.
Enzymatic disaggregation of cells can be done using either warm trypsinization or cold trypsinization. Since it is important to reduce the contact of cells to active trypsin so as to preserve maximum cell viability. Thus when entire tissue is trypsinized at 37°C cells which are dissociated should be collected after every half hour and then the trypsin should be removed by centrifugation and neutralized with serum in medium. In warm trypsinization, incubation is done for longer period of time and repeated sampling is required whereas cold trypsinization requires overnight, cold and short incubation. Trypsinization often permits the passage of cells to a new container which can be observed for experimentation and for reduction of the degree of confluency in the flask by removing a percentage of the cells. When the process is completed the cells appear rounded and will remain in suspension. Thus the process of trypsinization has a significant role in animal cell culturing and tissue culture technology.
Global Market Report of Trypsin (CAS 9002-07-7) aims at providing comprehensive data on Trypsin globally and regionally (Europe, Asia, North America, Latin America etc.). It captures Trypsin market trends, pays close attention to Trypsin
This report focuses on three primary areas; manufacture methods & technology development, market situation & trend analysis, and distribution policy.
The first section centers on manufacture methods & technology development and the main manufacture methods of are introduced. The detailed production techniques section covers technical principle, technical flow, technical process, facilities, post allocation, cost estimation, environmental protection, technical features, product quality standards and project feasibility study conclusions.
The second section focuses on the market situation & trend analysis. It looks at application and end products, the production situation, manufacturers & capacity statistics, market supply status & trend forecast, production statistics & trend analysis, downstream consumer market analysis, supply & demand analysis, price analysis, and import & export situation for the chemical in question.
The final section looks at the distribution policy; it looks at the major use segments, the consumers and the traders for the chemical in question.
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This page was last updated on 12th Sep, 2015
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