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As per available reports about 10 relevant journals, 15 Conferences, 30 workshops are presently dedicated exclusively to mutant selection and about 2,070 articles are being published on Mutant selection.
Replica plating has become an important technique of microbial genetics. It is useful in the screening for mutants that fail to grow under the selective regime. The position of an absent colony on the replica plate is used to retrieve the mutant from the master. For example replica plating can be used to screen auxotrophic mutants in this way. In general, replica plating is a way of retaining an original set of strains on a master plate while simultaneously subjecting replicas to various kinds of tests on different medias or under different environmental conditions.
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Scope and Importance:
Genome is not a static entity. It is subjected to different types of heritable changes. A sudden, heritable change in the sequence of an organism’s genome that gives rise to alternate forms of any gene is called as mutation. Selection of mutants from the wild type phenotype is done by replica plating technique. Replica plating is used for the detection of mutants, cell are transformed on to successive plates containing either a selective medium or a non selective medium. Microbial colonies form on the non selective plate in the same pattern as on the master plate. Only mutant cells can grow on the selective plate; the mutant colonies that are formed derive from colonies on the master plates that are mutant.
Microbial transformation is defined as the biological process of modifying an organic compound into a reversible product. It involves the use of chemically defined enzyme catalyzed reactions in the living cells. The advantage of biotransformation processes over chemical processes is that they provide high specificity to attack exact site on the substrate and for a single isomer of the product. Chemical reactions involved in microbial transformations include oxidation, reduction, acylation, phosphorylation, methylation, hydrolysis, amination, halogenations, glycosylation. One of the major applications of microbial transformation is in the production of secondary metabolites. Microbial biocatalysts have significant applicability in the methodological development of novel immobilization techniques.
The major product class of fermentation chemicals consists of alcohols, enzymes and organic acids. Fermentation chemicals are utilized in variety of applications in a wide range of chemical processes in industries such as alcohol industry, pharmaceutical industry, food and beverages industry, chemical industry, textile industry and rubber industry among others. The revenue generated by the global fermentation chemicals market was USD 41,567.7 million in 2012 which is expected to reach USD 60,123.9 million by 2019, growing at a CAGR of 5.4% from 2013 to 2019.
Relevant Society and Associations
1.American Society for Biochemistry and Molecular Biology
2.ASCPT American Society for Clinical Pharmacology and Therapeutics
3.ASGT American Society of Gene Therapy
4.European Association of Pharma Biotechnology
5.European Biopharmaceutical Enterprises
6.European Federation of Pharmaceutical Industries and Associations
7.Japan Bioindustry Associatio
2.A & V Envirotech
3.General Environmental Science Corp
8.Genesis Biosciences, Inc.
13.Eli Lilly & Co
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This page was last updated on 12th Sep, 2015
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