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As per available reports about 10 relevant journals, 15 Conferences, 30 workshops are presently dedicated exclusively to Heterologous Gene Expression and about 2,070 articles are being published on Heterologous Gene Expression.
Heterologous expression refers to the expression of a gene or part of a gene in a host organism, which does not naturally have this gene or gene fragment. Insertion of the gene in the heterologous host is performed by recombinant DNA technology. Analysis of gene function is of central importance for the understanding of physiological processes. Expression of genes in heterologous organisms has allowed the isolation of many important genes (e.g. for nutrient uptake and transport) and has contributed a lot to the functional analysis of the gene products. For animal research, expression in Xenopus oocytes and cell cultures are predominant techniques, whereas in the plant domain, yeast has become the prevalent expression system. This review provides a survey of this quickly developing field and intends to assist researchers in determining appropriate experimental approaches for specific biological questions. Because heterologous expression technology is of special value for the analysis of proteins that are difficult to handle biochemically, the examples given concentrate on membrane proteins, i.e. transporter proteins.
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Scope and Importance
Expression of a foreign protein in a heterologous host is a keystone of proteomics research and of the biotechnology industry. The ideal host should possess the ability to express any protein with relevant post-translational modifications such as sumoylation. Studies have reported that the simplest host cell i.e. Escherichia coli presents many challenges including protein misfolding, protein degradation, and low solubility during heterologous gene expression. The fusion technology can overcome the problems of protein solublities and misfolding and therefore is the ideal approach for enhanced heterologous protein expression and purification.
Heterologous Gene Expression is defined as the expression of a gene into a host organism using recombinant DNA technology. The host into which the gene is inserted should not naturally possess this gene or gene fragment. Recombinant DNA technology refers to molecular cloning techniques that involves joining together of DNA molecules from two different species which are then inserted into a host organism to produce new genetic combinations. Recombinant DNA is widely used in biotechnology industry, medicine and biomedical research. The host cell used for heterologous gene expression should show rapid growth, could be able to utilise the simple and inexpensive culture media. Yeast cells such as Saccharomyces cerevisiae and Pichia pastoris are the attractive hosts for creation of foreign proteins. The main advantages offered by these systems are the strongly developed and easily reachable genetic tools, rapid growth and also most of the cellular and metabolic processes which are established in higher eukaryotes are conserved in both of these yeast species.
OMICS International hosts over 400 leading-edge peer reviewed Open Access Journals and organize over 300 International Conferences annually all over the world. OMICS Publishing Group journals have over 3 million readers and the fame and success of the same can be attributed to the strong editorial board which contains over 30000 eminent personalities that ensure a rapid, quality and quick review process. ConferenceSeries signed an agreement with more than 500 International Societies to make healthcare information Open Access. OMICS International Organizes 300+ Conferences Every Year across USA, Europe & Asia with support from 1000 more scientific societies and Publishes 500+ Open access journals which contains over 30000 eminent personalities, reputed scientists as editorial board members.
Market Analysis
Over the years, the demand for gene expression has increased significantly owing to the increasing research on diseases like cancer, Alzheimer’s, and Parkinson’s. The technological advancements in the field of gene expression are expected to drive the global market in the coming years. Furthermore, advancements in sequencing platforms have also led to the decrease in the cost of sequencing. This is expected to boost the market as the amount of sequencing is likely to increase.
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This page was last updated on March 28, 2024