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Fluorescence imaging technology has the capability for real-time quantitative measurements that allow integration of anatomic, functional, and statistical data. Its contributions focus on studies of intracellular trafficking, epithelial function and viral gene delivery systems. This illustrates the capabilities of different modes of fluorescence imaging in addressing questions in cell biology and physiology research and also highlights their applications in pharmaceutical research.
There are three major types of fluorescence imaging, i.e. Fluorescence correlation spectroscopy, wide-field fluorescence microscopy and confocal laser scanning microscopy (CLSM). CLSM uses various techniques like fluorescence recovery after photo bleaching (FRAP), fluorescence loss in photobleaching (FLIP), photoactivation, fluorescence resonance energy transfer (FRET), fluorescence lifetime imaging, and total internal reflection fluorescence microscopy.
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Fluorescence microscopy was applied to investigate the capture of cell-penetrating TAT peptide-modified liposomes by various cells and stability and intracellular trafficking of captured TAT-liposomes inside cells. It was also used to confirm the successful transfection of cells with TAT-liposomes bearing the plasmid encoding for Green Fluorescent Protein (GFP). CLSM technique was found to be effective in examining the behaviour of an enhancer in Transdermal Drug Delivery Systems (TDDS) of the drug-in-adhesive type. However, it is noteworthy that many of the informative studies have exploited experimental approaches coupling CLSM with other imaging (or related) techniques such as that of FRET. In particular, its application at the cellular level is rapidly developing and should provide a large amount of important and new information. The future is sure to see fluorescence imaging, especially CLSM, which plays a decisive role in drug delivery research.
A drug is in the broadest of terms, a chemical substance that has known biological effects on humans or other animals. Foods are generally excluded from this definition, in spite of their physiological effects on animal species. In pharmacology, a drug is "a chemical substance used in the treatment, cure, prevention, or diagnosis of disease or used to otherwise enhance physical or mental well-being. “Drugs may be used for a limited duration, or on a regular basis for chronic disorders. Recreational drugs are chemical substances that affect the central nervous system, such as opioids or hallucinogens. Alcohol, nicotine, and caffeine are the most widely consumed psychotropic drugs worldwide.
A drug is any chemical you take that affects the way your body works. Alcohol, caffeine, aspirin and nicotine are all drugs. A drug must be able to pass from your body into your brain. Once inside your brain, drugs can change the messages your brain cells are sending to each other, and to the rest of your body. They do this by interfering with your brain's own chemical signals: neurotransmitters that transfer signals across synapses.
A drug is a chemical substance that can change how your body and mind work. Drugs of abuse are substances that people use to get high and change how they feel. They may be illegal drugs like pot, cocaine, or heroin. Or they may be legal for adults only, like alcohol and tobacco. Medicines that treat illness can also become drugs of abuse when people take them to get high not because they're sick and following their doctor's orders. People can even abuse cough or cold medicines from the store if they ignore the directions and take too much at one time.
The market, which includes the global and regional breakdown of sales data, market forecasting, available drug discovery technologies in the market, application of the technologies in commercial and research practice, regulatory aspects, patent analysis, market leaders and market share and so forth for the drug discovery technologies.
The report offers a summary of the global drug discovery technologies market along with region-wise sales analysis of regions such as North America (the U.S. and Canada), Europe and emerging markets (Asian countries, South America, parts of Africa, Russia etc.). The application of drug discovery technologies is based on the commercial and R&D uses of those technologies/products/platforms. The sales data for the global and regional markets were substantiated for the present and projected values through statistical analysis. The technology details in the report are based on their present/prospective application and expediency. A look at the current state of the drug discovery market, existing platforms and products on the market, and detailed analysis of the competitive environments including new, potential venues for novel technologies and approaches. Analyses of the main positive and negative factors in each sector of the market, potential future trends, and emerging technologies accross different segments of the industry.
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