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DNA sequencing is the method of determining the exact order of nucleotides within a DNA molecule. It contains any method or technology that is used to determine the order of the four bases—adenine, guanine, cytosine, and thymine—in a strand of DNA. The beginning of express DNA sequencing methods has greatly enhanced biological and medical research and discovery. .
Knowledge of DNA sequences has become necessary for basic biological research, and in numerous realistic fields such as diagnostic, biotechnology, forensic biology, virology and biological systematics. The fast speed of sequencing reached with modern DNA sequencing technology has been implementing in the sequencing of complete DNA sequences, or genomes of numerous types and species of life, including the human genome and other complete DNA sequences of many animal, plant, and microbial species. An example of the results of automated chain-termination DNA sequencing. The first DNA sequences were attained in the early 1970s by academic researchers using laborious methods based on two-dimensional chromatography.
DNA sequencing may be used to examine the sequence of individual genes, larger genetic regions (i.e. clusters of genes or operons), full chromosomes or entire genomes. Sequencing provides the order of individual nucleotides in DNA or RNA (commonly represented as A, C, G, T, and U) extracted from cells of animals, plants, bacteria, archaea, or almost any other source of genetic information. This is useful in Molecular biology, Evolutionary biology , Metagenomics , Forensic identification , Parental testing , Detect the presence of known genes for medical purposes .Basic methods of DNA sequencing are Maxam-Gilbert sequencing and Chain-termination method. Allan Maxam and Walter Gilbert issued a DNA sequencing method in 1977 created on chemical modification of DNA and subsequent cleavage at specific bases. Also known as chemical sequencing, this method permitted purified samples of double-stranded DNA to be used without further cloning. This method's use of radioactive labeling and its technical complexity depressed extensive use after refinements in the Sanger methods had been made. The chain-termination method established by Frederick Sanger and coworkers in 1977 has become the demanded method of choice, owed to its relative comfort and reliability. When created, the chain-terminator method used fewer toxic chemicals and lower amounts of radioactivity than the Maxam and Gilbert method. Because of its comparative comfort, the Sanger method was soon mechanized and was the method used in the first generation of DNA sequencers. Sanger sequencing is the only method which prevailed from the 80's until the mid-2000s. Over that time, great improvements were made in the technique, such as fluorescent labeling, capillary electrophoresis, and general automation.
OMICS Group is one of the renowned Open Access publishers is proud to serve the scientific community with a team of 20 k Editorial board members, close to 300 Open Access Journals and grounding close to century platforms for upcoming and ongoing research hits in mean with conferences. In addition OMICS too provides a rapid review process of 21 days with the reading number touching 2 Million. What else it requires if scientific community could find a single platform where the presented research is easily published, OMICS serves the same duo. Each and every research effort has a scientific concept behind it which needed to be upfronted to the scientific community to increase the probabilistic discovery from conceptualization of scientific ideas.
National Symposium and Workshop
EMBO Symposium — Long regulatory RNAs , 13 Sep 2014 → 18 Sep 2014, Poland
Workshop on Mathematics for Life Sciences, 14 Sep 2014 → 16 Sep 2014, Algeria
IEEE Cluster Workshop: PBio 2014, 26 Sep 2014, Madrid, Spain
The 2nd International Workshop on Big Data in Bioinformatics and Healthcare Informatics, 27 Oct 2014, United States
EMBL-EBI-Wellcome Trust workshop on Proteomics Bioinformatics, 10 Nov 2014 → 14 Nov 2014, United Kingdom
NGS 2014 Nordic — Next Generation Sequencing Symposium, 20 Nov 2014, Denmark
BioInfoSummer 2014: Summer Symposium in Bioinformatics, 01 Dec 2014 → 05 Dec 2014, Australia
Pacific Symposium on Biocomputing, 04 Jan 2015 → 08 Jan 2015, United States
Keystone Symposia: Precision Genome Engineering and Synthetic Biology, 11 Jan 2015 → 16 Jan 2015, USA
Association of forensic DNA analysts and administrators
NP society of DNA
International Livestock Research Institute (ILRI), Nairobi
Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India
Institute of Molecular Biology, Hong Kong
European Molecular Biology Lab (EMBL), Heidelberg, Germany
Imperial College Genetics and Genomics Research Institute, Hammersmith Hospital, London
National Human Genome Research Institute, USA
Commonwealth Scientific and Industrial Research Organization, Australia
National Institute of Genetics, Japan
International Conference on Molecular Genetics and Microbiology, London (Sep 26-27, 2014)
International Conference on Bioinformatics and Biomedical Science, Madrid (Nov 10-11, 2014)
International Conference on Bioinformatics and Molecular Biology, London(Aug 21-22, 2014)
International Conference on Computational and Systems Biology, Kuala Lumpur (Aug 25-26, 2014)
International Conference on Cellular and Molecular Bioengineering, Osaka (Oct 12-13, 2014)
International Conference on MicroRNAs and Single Molecule Biology, Singapore ,(Jan 08-09, 2015)
OMICS,4th International Conference and Exhibition on Cell & Gene Therapy, August 10-12, 2015 UK
International Conference on Molecular Biology, Biochemistry and Biotechnology, Dubai, (Jan 30-31, 2015)
Applera, Norwalk, Connecticut, United States
Applied Biosystems, Foster City, California
Asper Biotech, Estonia
Celera Genomics, USA
Genentech, San Francisco, CA
Genetix, Hampshire, UK
GenePeeks, New York
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This page was last updated on January 18, 2020