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ANANBIOANAL - 2010
Pharmaceutical R & D Summit
Determination of Meloxicam and Flufenamic Acid in
Pharmaceutical Formulations and Biological Fluids using
lanthanide Sensitized Luminescence
Salma M. Z. Al-Kindy, Fakhr Eldin O. Suliman, Salim Al-Habsy, Haider Al-Lawati
Department of Chemistry, College of Science, Sultan Qaboos University, Sultanate of Oman
T
wo highly selective and sensitive luminescence methods for the assay of anti-
inflammatory drug meloxicam (MX) and flufenamic acid (FFA) in biological fluid
and tap water are described. The assay of MX was based on europium sensitized
luminescence. The method makes use of radiative energy transfer from the enolate
ring to europium ions in methanol and in aqueous system. Optimum conditions for
the formation of the enolate Eu3+
Eu-MX complex was found to depend on the concentration of Tris buffer and Eu3+
complexes were investigated. In methanol, the
.
In aqueous system, maximum sensitization was obtained in the presence of 0.28%
Tween –80, 0.01M tris buffer pH 8.0, 6 mM of 1, 10- phenanthroline, 1.75 mM Eu3+
and
7 mM of gadolinium ions as co-luminescence reagent. Under the optimum conditions
linear calibration curves between 0-1000 and 0-800 ppb for MX in methanol and in
aqueous medium respectively were obtained with the detection limit being 6.0 ppb.
The proposed method was successfully applied for the determination of MX in Mobic
and Co-Oxicam tablets and in tap water and urine samples. Excellent recovering
were obtained for the MX samples. The second method, a novel sequential injection
analysis (SIA) approach was used for the determination of FFA in samples of urine
and tap water. The method was based luminescence sensitization of terbium by
complex formation with FFA. The luminescence signal was monitored at lem
when excited at lex
doi:10.4172/2155-9872.1000093
= 565nm
= 298nm using time resolved mode. Experimental factors that
influenced fluorescence reaction were systematically optimized in aqueous medium
using chemometric optimization. Under the optimum conditions linear calibration
curves between 0-1200 ppb for FFA in aqueous medium were obtained with a
LOD of 80 ppb. When applied to Urine and Tap water samples the procedures
were found to be free from matrix interferences except for Fe3+ that had significant
interference effect. The results obtained for the assay of FFA in urine and tap water
samples demonstrated good accuracy and precision.
ANALBIOANAL-2010
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