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ANANBIOANAL - 2010
Pharmaceutical R & D Summit
doi:10.4172/2155-9872.1000089
Comparision of Retinol Plasma Levels in Patients with Bladder
Cancer and Healthy Volunteer with HPLC-DAD Methods after a
Single Oral b-Carotene Administration
Yucel Kadioglu, Fatma Demirkaya
Atatürk University, Faculty of Pharmacy, Erzurum, Turkey
T
he disease-preventing activity of b-carotene could be ascribed either to their
conversion into retinoid or to their activity as intact molecules.The aim of study
were to develop and validate a simple, precise, accurate and specific HPLC-DAD
method for determination of retinol from human plasma and to examine whether retinol
levels of patients with bladder cancer are increased or decreased when compared
to healthy volunteers after a single oral administration of 20 mg of b-carotene. The
chromatographic conditions of the HPLC-DAD methods using vitamin K2
as the
internal standard (IS) were optimized. Retinol and IS were extracted into n-hexane
and chloroform containing butylated hydroxytoluene solvent system.The method
has a wide linear over the 0.5-10 µg/mL of concentration range (the endogenous
retinol has a concentration of approximately 0.79µg/mL). The precision (RSD %) of
this method was less than 7.9%, and accuracy (RE) was better than ± 9.1 (n=6).
The developed and validated method could be successfully applied to determination
of retinol measured in plasma samples (one milliliter blood samples were collected
at 0 (before dosing) and 2.5 h after dosing) from six healthy volunteers and six
bladder cancer patients following oral administration of single dose of b-carotene.
Obtained data in this study were compared by Student-t test (at 95% coeffidence
level). There is no significant difference between plasma concentration of retinol of
healthy volunteers and bladder cancer patients (for 0 h; th
th
=0.208; P>0.05).
=1.374; P>0.05, for 2.5 h;
ANALBIOANAL-2010
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