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ANANBIOANAL - 2010
Pharmaceutical R & D Summit
doi:10.4172/2155-9872.1000086
Using ATR- FTIR for Detection of Immobilized Biomolecules on
Amino Acid Functionalized HAp Nanoparticles
Renu Sharma, Ravi Ranjan Pandey, Satabhisa Kar, Marshal Dhayal
Centre for Cellular and Molecular Biology, Hyderabad, India
S
urface functionalization nanoparticles (size <100 nm) with bioactive molecules is
a rapidly expanding field in current biomaterial research and it should be of great
interest to fine-tune the bioactivity of such nanoparticles by surface functionalization
using water-soluble biomolecules. Calcium hydroxyapatite or calcium phosphate
(HAp, Ca10
the bones and teeth has been used as a model system. In this regard, amino acids
were selected as ideal candidates for surface functionalization for production of
bioinorganic HAp nanoparticles and bionanocomposites due to their relative low
cost, intrinsic biocompatibility and ability to interact with HAp surfaces. This paper
highlights, in the first part, synthesis of amino acid-functionalized hydroxyapatite
(HAp-AA) nanoparticles with uniform size and having rod-like morphology which
was achieved by wet chemical process with Ca(OH)2
(PO4
)6
(OH)2
transform infrared-attenuated total reflectance (FTIR-ATR) spectroscopy had been
used to quantify to determine carboxylate group of the amino acid which was
preferentially bound to the surfaces of hydroxyapatite nanoparticles. Variations in
the size and shape of the HAp nanoparticles functionalized with different amino
acid were consistent with differences in the strength of binding at the HAp surfaces.
Second part of the paper describes enzyme immobilization on these functionalized
HAp nanoparticles and FTIR-ATR spectroscopy was used for detection of relative
proportion of enzyme loading on HAp.
: H3
PO4
: amino acid. Fourier
) nano-particles which is the main inorganic constituent of
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