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ANANBIOANAL - 2010
Fabrication of Novel Nanostructured Pd2+
for Detection of Cholesterol
Marshal Dhayal1
,Ravi Ranjan Pandey1
, Krishan Kumar Saini2
1Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad, India
2National Physical Laboratory, Dr K S Krishnan Marg, New Delhi, India
W
ith recent advances in nanotechnology, rapid progress has been made in
biosensors based on nanomaterials, however there are still challenges to
overcome with practical applicability of such systems. We describe nanomaterial-
based biosensors with unique properties, provides a promising platform, which is
simple, cost-effective, and requires no external modification to biomolecules. The
unique catalytic, amperometric cholesterol biosensors have been developed based
on the immobilization of cholesterol oxidase (ChOx) in the nonostructured films of
sol–gel-derived Pd doped TiO2
ITO providing more surface energy as well more surface area which improves the
sensitivity and long-term stability of the biosensor. Analytical performance of the
cholesterol biosensor based on the Pd doped TiO2
biosensor based on undoped TiO2
long-term stability. The Pd doped TiO2
. The presence of Pd in the sol–gel-derived TiO2
direct electron transfer between the electrode surface and the active redox centers
of Cholesterol oxidase (ChOx) which enables the biosensor to operate at a low
working potential and to avoid the influence of the O2
current response. This work offers a unique platform for development of enzymatic
electrochemical biosensors.
presence on the amperometric
onto
films is superior to that of the
films in terms of response time, sensitivity, and
nanostructured platform offer the pathway for
Pharmaceutical R & D Summit
doi:10.4172/2155-9872.1000084
Doped Tio2
Platform
ANALBIOANAL-2010
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