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ANANBIOANAL - 2010
Pharmaceutical R & D Summit
doi:10.4172/2155-9872.1000083
Application of Enzyme Immobilized Nanostructured Fe doped
TiO2
Ravi Ranjan Pandey1
for Electrochemical Quantification of Urea
, Krishan Kumar Saini2
, Marshal Dhayal1
1Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad, India
2National Physical Laboratory, Dr K S Krishnan Marg, New Delhi, India
E
lectrochemical enzymatic biosensors detect their targets based on enzymatic
catalysis of a reaction that produces or consumes electrons. Electron
communication between the enzymes and the electrode is a major barrier in the
development of electrochemical enzymatic sensors. One of the reasons for this is
that the active redox centers of enzymes are deeply embedded in an electrically
insulate protein shell. In order to address this problem, we have utilized Fe doped
metal oxide (TiO2
) films as the shuttles to transport electrons between the electrode
and the redox centers of enzymes. An electrochemical biosensor was fabricated for
the quantitative determination of urea in aqueous medium using PBS, at pH-7.2.
The urease (Urs) was immobilized onto an electrode made of Fe doped TiO2
films
onto an indium-tin oxide (ITO) coated glass substrate using sol-gel technique. The
linkage between the Urs enzyme and Fe doped TiO2
enzyme electrode (Urs/Fe-TiO2
/ITO) with a high level of enzyme immobilization
and excellent lifetime stability. The response studies were carried out as a function
of urea concentration with amperometric measurements. The biosensor based on
Urs/Fe-TiO2
/ITO as the working electrode showed an enhanced sensitivity of urea,
indicating the Urs enzyme immobilized on the electrode surface had a high affinity
to urea.
films provided the resulting
ANALBIOANAL-2010
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