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ANANBIOANAL - 2010
Pharmaceutical R & D Summit
doi:10.4172/2155-9872.1000074
Development of a Dispersive Micro-Liquid Liquid Method for the
Extraction of Pyrethroid Pesticides from Environmental Matrices
for Determination by Reversed-Phase High Performance Liquid
Chromatography
Saeed S. Albaseer1
Hyderabad, India
2
, R. Nageswara Rao2
, Y. V. Swamy3
, K. Mukkanti1
1Centre for Chemical Sciences and Technologies, Institute of Science and Technology, Jwaharlal Nehru Technological University,
HPLC group, Analytical Chemistry Division, Indian Institute of Chemical Technology, Hyderabad, India
3Bioengineering and Environmental Centre, Indian Institute of Chemical Technology, Hyderabad, India
T
he conventional multiresidue methods involve the consumption of large
quantities of toxic organic solvents which necessitate further clean up steps
before analysis. In addition, the use of large amounts of toxic solvents for sample
preparation is not eco-friendly and difficult to justify. Miniaturization can serve well
in this regard and is currently a major trend in analytical chemistry. In the present
work we developed a method for the rapid trace analysis of residual pyrethroid
pesticides in agricultural waters by high performance liquid chromatography with
diode array detection using dispersive liquid–liquid microextraction (DLLME). To the
best of authors’ knowledge, this is the first application of DLLME for the extraction of
pyrethroid pesticides from aqueous matrices. Several parameters of the extraction
procedure such as type and volume of extraction solvent, type and volume of
dispersive solvent and salt addition were evaluated to achieve the highest yield
and to attain the lowest detection limits. The DLLME procedure optimized consists
in the formation of a cloudy solution promoted by the fast addition to the sample
(5 ml) of a mixture of carbon tetrachloride (extraction solvent, 55 µL) and acetone
(dispersive solvent, 500 µL). The tiny droplets formed and dispersed among the
aqueous sample solution are further joined and sedimented (25 µL) in the bottom of
the conical test tube by centrifugation. Once extracted, the pesticides were directly
injected and separated by RP-HPLC comprising a short column (Hiber- purospher
star RP-18 end capped column (150 x 4.6 mm I.D., 5 μm particle size) and a PDA
detector.
ANALBIOANAL-2010
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