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ANANBIOANAL - 2010
Pharmaceutical R & D Summit
doi:10.4172/2155-9872.1000077
Solid-Phase Extraction of Tramadol from Plasma and Urine
Samples Using a Novel Water-Compatible Molecularly Imprinted
Polymer
Mehran Javanbakht1
, Abdol Mohammad Attaran2
, Mohammad Hadi Namjumanesh1
1Department of Chemistry, Amirkabir University of Technology, Tehran, Iran
2Department of Chemistry, Payamenoor University, Delijan, Iran
3Food & Drug Laboratory Research Center, Food & Drug Department, Ministry of Health and Medical Education, Tehran, Iran
I
n this study, a novel method is described for the determination of tramadol in
biological fluids using molecularly imprinted solid-phase extraction (MISPE) as the
sample clean-up technique combined with high-performance liquid chromatography
(HPLC). The water-compatible molecularly imprinted polymers (MIPs) were prepared
using methacrylic acid as functional monomer, ethylene glycol dimethacrylate as
cross-linker, chloroform as porogen and tramadol as template molecule. The novel
imprinted polymer was used as a solid-phase extraction (SPE) sorbent for the
extraction of tramadol from human plasma and urine. Various parameters affecting
the extraction efficiency of the polymer have been evaluated. The optimal conditions
for the MIP cartridges were studied. The MIP selectivity was evaluated by checking
several substances with similar molecular structures to that of tramadol. The limit of
detection (LOD) and limit of quantification (LOQ) for tramadol in urine samples were
1.2 and 3.5 μg L-1
3.0 and 8.5 μg L-1
higher than 91%.
, Behrouz Akbari-adergani3
, respectively. These limits for tramadol in plasma samples were
, respectively. The recoveries for plasma and urine samples were
ANALBIOANAL-2010
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