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ANANBIOANAL - 2010
Pharmaceutical R & D Summit
doi:10.4172/2155-9872.1000038
Role of Igf Signaling in Prostate Cancer Cell Line (Pc-3)
J. Arunakaran
Department of Endocrinology, Dr. ALM. Post Graduate Institute of Basic Medical Sciences, University of Madras, Chennai, India
H
uman prostate carcinoma is one of the most common cancers among men and
second leading cause of cancer related death. Prostate cancer cell growth is
controlled by several factors including androgen, growth factors and their respective
receptors. Growth factors and their receptors are over expressed in advanced
prostate cancer including epidermal growth factor (EGF), transforming growth
factors (TGF) α and β, fibroblast growth factors (FGF), and insulin like growth
factors (IGFs). IGFs are potent mitogenic factors for a variety of cancer including
prostate cancer which stimulates cell growth and inhibits apoptosis. The importance
of the IGF system in prostate cancer cell growth is underscored by the detection of
every molecules of this system, including IGF-I, IGF-II, IGF-IR and as well as IGF
binding proteins in normal, hyperplastic and or neoplastic cells and tissues. IGFBP3
binds to IGF-I or IGF-II and blunts their proliferative effects on cells. IGF-I increases
proliferation of prostate cancer cells whereas antisense mediated inhibition of IGF-
IR expression suppresses in vivo tumor growth and prevents prostate cancer cell
invasiveness. Quercetin which possesses a wide spectrum of pharmacological
properties is found in many fruits and vegetables. It inhibits the proliferation of cancer
cells and induces apoptosis. Our previous studies proved that quercetin leads to cell
cycle arrest and induces apoptosis in prostate cancer cells. We also studied the
effect of quercetin on IGF system and its signaling molecules of prostate cancer.
Zinc is an essential trace element present in mammalian prostate gland. Our recent
study demonstrated that zinc treatment significantly reduces the cell viability of PC-3
cells. Zinc leads to cell cycle arrest and induces apoptosis by regulating IGF system
and signaling molecules. It decreases the protein levels of IGF-IR, IRS-I, IRS-2 and
increased the level of IGFBP-3. Thus, our study suggests that quercetin and zinc
decreases the survival of androgen independent prostate cancer cells by modulating
the expression of IGFs systems, signaling molecules and induces apoptosis which
could be useful for the androgen independent prostate cancer treatment.
ANALBIOANAL-2010
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