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ANANBIOANAL - 2010
Pharmaceutical R & D Summit
doi:10.4172/2155-9872.1000021
Role of Non HLA Genetic Variants in End Stage Renal Disease
Suraksha Agrawal
Department of Medical Genetics, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow,
India
C
ytokines and intercellular adhesive molecules (ICAM) play a crucial role in the
pathogenesis of primary kidney disease and progression to end stage renal
disease (ESRD). Cytokine secretion is reported to be dependent upon the SNP’s
located in the cytokine genes. The role of different polymorphisms of cytokines and
ICAM genes as a probable susceptibility factors for ESRD have been explored in
the present study.
The study was conducted on 258 ESRD patients and ethnically matched 569
controls. Individuals were genotyped for IL-6 (G174C), IL-4 (C590T), TNF-α (-G308A
and -G238A) and ICAM-1 (A469G) gene polymorphisms using standard PCR-RFLP
based method.
We observed significant difference in the genotype frequencies of the TNF-α
-308AA (p=0.001, OR=7.61, 95%CI=2.1-27.9), TNF-α -238AA (p=0.001, OR=5.8,
95%CI=2.2–15.1). Further, C allele of IL-6 -G174C and G allele of ICAM-1 A469G
were significantly different in ESRD patients when compared to controls (p=0.0001;
OR=5.5, 95%CI=3.9-7.7 and p<0.0001; OR=3.8, 95%CI=3.1-4.7). For the IL-4
C590T polymorphism, though the homozygous mutant genotype (TT) was not found
to be significantly associated with ESRD, a statistically significant association with T
allele (p=0.0001) was found with the ESRD. Further, combined analysis revealed
a higher risk in ESRD patients with low IL-4 and high IL-6 producing genotypes and
high producing genotype of TNF-α (308 and 238) with the increased risk of ~6.0 fold
and 3.3 fold respectively. Our results suggest that IL-6, IL-4, TNF-α and ICAM gene
polymorphism may be risk factors for ESRD.
ANALBIOANAL-2010
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