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Recommended Conferences for Trypsinization

Trypsinization


As per available reports about 10 relevant journals, 15 Conferences, 30workshops are presently dedicated exclusively to Trypsinization and about 2,070 articles are being published on trypsinization

Trypsinization is defined as the process of dissociating a cell using trypsin. Trypsin is a proteolytic enzyme which breaks down proteins and digests adherent cells in the vessel in which they are cultured. Upon addition to animal cell culture, it breaks down the proteins and thus enabling the cells to get adheres to the vessel. However cell surface proteins are cleaved sometimes due to the proteolytic activity of trypsin which leads to dysregulation of the cell functions. To overcome that it is necessary to add trypsin inhibitors like serum and divalent cations like calcium and magnesium can be added. Serum is usually added to the container once cells have detached and cell detachment can be confirmed by observing under a microscope.

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Scope and Importance:
Trypsinization is often done to permit passage of the cells to a new container, observation for experimentation, or reduction of the degree of confluence in the flask by removal of a percentage of the cells. This process of cell culture or tissue culture requires a method to dissociate the cells from the container and each other. Trypsin, an enzyme commonly found in the digestive tract, can be used to "digest" the proteins that facilitate adhesion to the container and between cells. For experimental purposes, cells are often cultivated in containers that take the form of plastic flasks or plates. In such flasks, cells are provided with growth medium comprising the essential nutrients required for proliferation, and the cells adhere to the container and each other as they grow.

Enzymatic disaggregation of cells can be done using either warm trypsinization or cold trypsinization. Since it is important to reduce the contact of cells to active trypsin so as to preserve maximum cell viability. Thus when entire tissue is trypsinized at 37°C cells which are dissociated should be collected after every half hour and then the trypsin should be removed by centrifugation and neutralized with serum in medium. In warm trypsinization, incubation is done for longer period of time and repeated sampling is required whereas cold trypsinization requires overnight, cold and short incubation. Trypsinization often permits the passage of cells to a new container which can be observed for experimentation and for reduction of the degree of confluency in the flask by removing a percentage of the cells. When the process is completed the cells appear rounded and will remain in suspension. Thus the process of trypsinization has a significant role in animal cell culturing and tissue culture technology.

Market Analysis:

Global Market Report of Trypsin (CAS 9002-07-7) aims at providing comprehensive data on Trypsin globally and regionally (Europe, Asia, North America, Latin America etc.). It captures Trypsin market trends, pays close attention to Trypsin

This report focuses on three primary areas; manufacture methods & technology development, market situation & trend analysis, and distribution policy.

The first section centers on manufacture methods & technology development and the main manufacture methods of are introduced. The detailed production techniques section covers technical principle, technical flow, technical process, facilities, post allocation, cost estimation, environmental protection, technical features, product quality standards and project feasibility study conclusions.

The second section focuses on the market situation & trend analysis. It looks at application and end products, the production situation, manufacturers & capacity statistics, market supply status & trend forecast, production statistics & trend analysis, downstream consumer market analysis, supply & demand analysis, price analysis, and import & export situation for the chemical in question.

The final section looks at the distribution policy; it looks at the major use segments, the consumers and the traders for the chemical in question.

List of Best International Conferences

  1. 6th Proteomics Conference and  Expo, March 29-30, 2016, Atlanta, USA
  2. Structural Biology Conference, June 23-24, 2016, New Orleans, USA
  3.  Next Generation Sequencing Conference, July 21-22, 2016, Berlin, Germany
  4.  Nucleic Acids Conference, Aug 4-6, 2016, Seattle, USA
  5. 2nd Transcriptomics Conference, August 18-20, 2016, Portland, USA
  6. 5th Computational Systems Biology Conference, August 22-23, 2016, Philadelphia, USA
  7. 2nd Glycobiology Congress, August 29-31, 2016, Atlanta, USA
  8. 2nd Lipid Science Conference, October 06-08, 2016, Miami, USA
  9. 7th Proteomics Conference, October 24-26, 2016, Rome, Italy
  10. Biochemistry Conference, October 13-15, 2016, Kualalumpur, Malaysia
  11. 7th Bioinformatics Conference, October 27-28, 2016, Chicago, USA
  12. 2nd Genetic and Protein Engineering Conference, November 14-16, 2016, Atlanta, USA
  13. Glycomics Conference, December 01-03, 2016, Chicago, USA
  14. Amino Acids and Proteins Congress, December 08-09, 2016, Baltimore, USA
  15. Protein Engineering Conference , October 26-28, 2015, Chicago, USA
  16. 5th Biodiversity Conference, March 10-12, 2016 Madrid, Spain
  17. 2nd Applied Microbiology Conference, October 31-November 02, 2016 Istanbul, Turkey
  18. Water Microbiology and Novel Technologies conference, July 18-20, 2016 Chicago, USA
  19. 7th Bioavailability and Bioequivalence Summit, August 29-31, 2016 Atlanta, USA
  20. Clinical and  Molecular Genetics Conference, November 28-30, 2016 Chicago, USA

Relevant Society and Associations

  1. European Association of Pharma Biotechnology
  2. European Biopharmaceutical Enterprises
  3. European Federation of Pharmaceutical Industries and Associations
  4. Japan Bioindustry Association
  5. American Society for Biochemistry and Molecular Biology
  6. ASCPT American Society for Clinical Pharmacology and Therapeutics
  7. ASGT American Society of Gene Therapy
  8. ASMUSA American Society for Microbiology
  9. All India Biotech Association
  10. Association of Biotechnology Led Enterprises
  11. Biotechnology Industry Organization

Companies

  1. Amgen
  2. Allergan
  3. GlaxoSmithKline
  4. Bayer
  5. Bristol-Myers Squibb
  6. Biogen Idec
  7. AbbVie
  8. Celgene
  9. AstraZeneca
  10. Eli Lilly & Co
  11. Abbott Laboratories
  12. Valeant Pharmaceuticals
  13. Johnson & Johnson
  14. Novartis
  15. Roche
  16. Pfizer
  17. Merck
  18. Novo Nordisk
  19. Gilead Sciences
  20. Sanofi

 

 

This page will be updated regularly.

This page was last updated on 12th Sep, 2015

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